Oleate Prevents Palmitate-Induced Atrophy via Modulation of Mitochondrial ROS Production in Skeletal Myotubes

Table 1

Primer sets for qRT-PCR.

Gene		Primer sequence	Gene		Primer sequence
Pgc-1α	Forward	TGATGTGAATGACTTGGATACAGACA	Opa1	Forward	GGTCACCACGAGAAATCTCAG
	Reverse	GCTCATTGTTGTACTGGTTGGATATG		Reverse	TCTTCCATTCCGTCTCTAGGTT
Ncor1	Forward	GACCCGAGGGAAGACTACCATT	Drp1	Forward	CGGGACAAGTTAATTCAGGACA
	Reverse	ATCCTTGTCCGAGGCAATTTG		Reverse	GTTCTCGGGCAGACAGTTTTC
Nrf1	Forward	GAACGCCACCGATTTCACTGTC	TNF-α	Forward	CACAAGATGCTGGGACAGTGA
	Reverse	CCCTACCACCCACGAATCTGG		Reverse	TCCTTGATGGTGGTGCATGA
Nrf2	Forward	GGCACAGTGCTCCTATGCGTG	IL6	Forward	CCACGGCCTTCCCTACTTC
	Reverse	CCAGCTCGACAATGTTCTCCAGC		Reverse	TTGGGAGTGGTATCCTCTGTGA
Tfam	Forward	CTGATGGGTATGGAGAAGGAGG	IL1-β	Forward	GCTCATCTGGGATCCTCTCC
	Reverse	CCAACTTCAGCCATCTGCTCTTC		Reverse	CCTGCCTGAAGCTCTTGTTG
Fis1	Forward	GCTCTAAAGTATGTGCGAGGG	Atrogin-1	Forward	CGTGCCGCCTGGAGAAAC
	Reverse	TGCCTACCAGTCCATCTTTCTT		Reverse	TGGGAGTTGCTGTTGAAGTCG
Mfn1	Forward	TTGATCGAATAGCATCCGAGGA	Murf1	Forward	TGAGGTGCCTACTTGCTCCT
	Reverse	CACAGCATTGCATTGATGACAG		Reverse	TCACCTGGTGGCTATTCTCC
Mfn2	Forward	GTGGGCTGGAGACTCATCG	Myostatin	Forward	TAACCTTCCCAGGACCAGGA
	Reverse	CTCACTGGCGTATTCCGCAA		Reverse	CACTCTCCAGAGCAGTAATT