Research Article

Aspalatone Prevents VEGF-Induced Lipid Peroxidation, Migration, Tube Formation, and Dysfunction of Human Aortic Endothelial Cells

Figure 6

Aspalatone inhibits VEGF-induced reactive oxygen species production in HAECs. (a) Flow cytometric analysis showing CM-H2DCFDA fluorescence in HAECs treated with VEGF (10 ng/mL) without or with aspalatone (50 μM) for 18 h (red: VEGF alone treated, pink: VEGF + aspalatone, green: control, and blue: aspalatone). Unfilled histograms with solid black lines were unstained controls. (b) Bars showing fold change in CM-H2DCFDA Mean Fluorescence Intensity (MFI) in HAECs treated with aspalatone (50 μM) in the absence or presence of VEGF (10 ng/mL) for 18 h. Bars represent mean ± SD (). when compared with control and when compared with VEGF-treated group.
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