Basic Fibroblast Growth Factor Inhibits Apoptosis and Promotes Proliferation of Adipose-Derived Mesenchymal Stromal Cells Isolated from Patients with Type 2 Diabetes by Reducing Cellular Oxidative Stress

Figure 5

Cellular senescence and apoptosis level in healthy and diabetic-ASCs cultured under standard conditions or exposed to various doses of bFGF. Viable and dead cells were visualized by simultaneous fluorescent staining with calcein-AM and propidium iodide solutions, respectively (a). Senescent cells in cultures were indicated by detection of senescence-associated β-galactosidase (b). Proportion of dead cells was calculated on the basis of the image analysis of at least 10 representative shots of calcein-AM/propidium iodide double staining (c); similarly, the numerousness of senescent cells was determined by quantification of SA-βgal stained area (d). Additionally, apoptotic cells were identified by immunofluorescent staining of active caspase-3; nuclei were counterstained with DAPI (e). p value <0.05, p value <0.01, and p value <0.001.

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