Table 4: Primers (SYBR® Green) targeting 16rRNA coding regions of bacteria, butyryl-coenzyme A (CoA) CoA transferase genes, and butyrate kinase gene.

Target organismPrimerSequence (5′-3′)Size (bp)Conc. [pmol/µL]Reference

LactobacilliFwd primerAGC AGT SGG GAA TCT TCC A352–7004 [19]
Rev primerATT YCA CCG CTA CAC ATG4

EnterobacteriaFwd primerAGC ACC GGC TAA CTC CGT492–5093 [20]
Rev primerGAA GCC ACG CCT CAA GGG CAC AA834–8563

PrevotellaFwd primerCACCAAGGCGACGATCA14582,5 [21]
Rev primerGGATAACGCCYGGACCT2,5

AkkermansiaFwd primerCAGCACGTGAAGGTGGGGAC15052,5 [22]
Rev primerCCTTGCGGTTGGCTTCAGAT2,5

BCoAT geneFwd primerGCIGAICATTTCACITGGAAYWSITGGCAYATG~54027 [23]
Rev primerCCTGCCTTTGCA ATRTCIACRAANGC27

Butyrate kinaseFwd primerTGCTGTWGTTGGWAGAGGYGGA27318 [24]
Rev PrimerGCAACIGCYTTTTGATTTAATGCATGG18