EGCG Prevents High Fat Diet-Induced Changes in Gut Microbiota, Decreases of DNA Strand Breaks, and Changes in Expression and DNA Methylation of Dnmt1 and MLH1 in C57BL/6J Male Mice

Table 4

Primers (SYBR® Green) targeting 16rRNA coding regions of bacteria, butyryl-coenzyme A (CoA) CoA transferase genes, and butyrate kinase gene.

Target organism	Primer	Sequence (5′-3′)	Size (bp)	Conc. [pmol/µL]	Reference
Lactobacilli	Fwd primer	AGC AGT SGG GAA TCT TCC A	352–700	4	[19]
	Rev primer	ATT YCA CCG CTA CAC ATG		4
Enterobacteria	Fwd primer	AGC ACC GGC TAA CTC CGT	492–509	3	[20]
	Rev primer	GAA GCC ACG CCT CAA GGG CAC AA	834–856	3
Prevotella	Fwd primer	CACCAAGGCGACGATCA	1458	2,5	[21]
	Rev primer	GGATAACGCCYGGACCT		2,5
Akkermansia	Fwd primer	CAGCACGTGAAGGTGGGGAC	1505	2,5	[22]
	Rev primer	CCTTGCGGTTGGCTTCAGAT		2,5
BCoAT gene	Fwd primer	GCIGAICATTTCACITGGAAYWSITGGCAYATG	~540	27	[23]
	Rev primer	CCTGCCTTTGCA ATRTCIACRAANGC		27
Butyrate kinase	Fwd primer	TGCTGTWGTTGGWAGAGGYGGA	273	18	[24]
	Rev Primer	GCAACIGCYTTTTGATTTAATGCATGG		18