Triethylenetetramine Synergizes with Pharmacologic Ascorbic Acid in Hydrogen Peroxide Mediated Selective Toxicity to Breast Cancer Cell

Figure 5

CAT expression levels in breast cancer cells and normal cells. (a) MCF-7 cells were treated with 1 mM Asc, 10 μM TETA, 300 U/mL catalase, 1 mM Asc/10 μM TETA, or 1 mM Asc/10 μM TETA plus catalase for 12 hours; cloning formation assay was performed, , , ; (b) CAT expression levels in HS578Bst and MCF-7 cells were determined by western blotting; (c) CAT expression in HS578Bst was suppressed by CAT shRNA; (d) HS578Bst cell viability after CAT shRNA treatment was measured by MTT assay, , .

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