Inhibition of Caveolae Contributes to Propofol Preconditioning-Suppressed Microvesicles Release and Cell Injury by Hypoxia-Reoxygenation

Figure 4

Caveolae inhibitor enhances the protective effects of propofol preconditioning against H/R-induced mitochondrial-dependent apoptosis and the release of EMVs. (a, b) The protective effects of propofol preconditioning against H/R-induced mitochondrial damage measured by mitochondrial viability and intracellular ATP content are enhanced by caveolae inhibitor. (c, d) The protective effects of propofol preconditioning against H/R-induced decrease of mitochondrial membrane potential measured by JC-1 stain and by flow cytometry. (e) The protective effects of propofol preconditioning against H/R-induced increase of mitochondrial membrane permeability are enhanced by caveolae inhibitor. (f–k) Detecting the expression level of CAV-1, Bax, BCL2, cytochrome c, caspase 3, COX IV, and their gray scales calculated by ImageJ software. (l) The protective effects of propofol preconditioning against H/R-induced release of EMVs are enhanced by caveolae inhibitor. Mean ± SD are from 5 different experiments. refers to , refers to .

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