Protection of Human Umbilical Vein Endothelial Cells against Oxidative Stress by MicroRNA-210

Figure 5

miR-210 reduced ROS generation in HUVECS treated with H₂O₂. (a) HUVECs were transfected with miR-210 mimics, miR-210 inhibitor, or NC and subsequently treated with H₂O₂ for 24 h (0, 0.5 mmol/L). Cells were collected and incubated with 10 μM DCFH-DA for 20 minutes according to the manufacturer’s instructions. After the incubation, the cells were washed with warm PBS, and ROS levels were analyzed by flow cytometry. (b) Quantification of mean fluorescent intensity in each group. Stars above bars refer to values between NC-transfected cells treated with H₂O₂ and the corresponding group. All , .

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