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Oxidative Medicine and Cellular Longevity
Volume 2017 (2017), Article ID 3631565, 14 pages
https://doi.org/10.1155/2017/3631565
Research Article

Hepatoprotective Effect of Polyphenol-Enriched Fraction from Folium Microcos on Oxidative Stress and Apoptosis in Acetaminophen-Induced Liver Injury in Mice

1Application Technique Engineering Center of Natural Cosmeceuticals, College of Fuijan Province, Xiamen Medical College, Xiamen, Fujian 361023, China
2Research Center of Natural Cosmeceuticals Engineering, Xiamen Medical College, Xiamen, Fujian 361023, China
3Fujian Provincial Key Laboratory of Biological Engineering on Traditional Herbs, Xiamen Medical College, Xiamen, Fujian 361023, China
4Technology and Engineering Center for Marine Biomedical Resource Utilization, Xiamen Medical College, Xiamen, Fujian 361023, China
5Department of Pharmacy, Xiamen Medical College, Xiamen, Fujian 361023, China

Correspondence should be addressed to Gang Zhang; nc.ude.cmmx@gz and Gueyhorng Wang; nc.ude.cmmx@hgw

Received 21 November 2016; Revised 24 February 2017; Accepted 20 March 2017; Published 23 May 2017

Academic Editor: Jie Li

Copyright © 2017 Hongtan Wu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

TABLE S1. In vitro antioxidant activities of different extracts from Folium Microcos. Figure S1: Effects of FMF on morphological changes induced by H2O2 in HepG2 cells. Cells were treated with FMF (10, 20, 40, 80, 100, and 200 μg/mL) in the presence of 400 μM H2O2 for 4 h and observed by microscope. (a) Control cells. (b) Cells exposed to H2O2. (c-h) Cells pretreated with different doses of FMF and then exposed to H2O2. Figure S2: Effects of FMF on H2O2-mediated oxidative stress in Hepa1-6 cells. Cells were treated with FMF (10, 20, 40, 80, 100, and 200 μg/mL) in the presence of 400 μM H2O2 for 4 h. (a) ROS formation was measured using a fluorescence microplate reader. (b) Cellular mortality was evaluated by MTT assay. Results are shown as mean ± SD (n=3). ∗∗∗ P < 0.001 compared with the control group; ### p < 0.001, ## p < 0.01, # p < 0.05 compared with H2O2-intoxicated group. Figure S3: Effects of FMF on morphological changes induced by H2O2 in Hepa1-6 cells. Cells were treated with FMF (10, 20, 40, 80, 100, and 200 μg/mL) in the presence of 400 μM H2O2 for 4 h and observed by microscope. (a) Control cells. (b) Cells exposed to H2O2. (c-h) Cells pretreated with different doses of FMF and then exposed to H2O2. Figure S4: Cytotoxicity assay. (a) HepG2 and (b) Hepa1-6 cells were treated with FMF (10, 20, 40, 80, 100, and 200 μg/mL) for 24 h, and cellular mortality was evaluated by MTT assay. Results are shown as mean ± SD (n=3). Figure S5: Effects of FMF on Nrf2 nuclear translocation and its target gene expression. Hepa1-6 cells were treated with FMF (100 μg/mL) for 12 and 24 h. (a) Nuclear and cytoplasmic extracts of cells were prepared, and the protein level of Nrf2 was determined by western blot. Lamin B and Tubulin were used as endogenous controls for nucleus and cytoplasm, respectively. (b)Total cellular protein was extracted, and protein levels of Nrf2, NQO1 and HO-1 were determined by western blot. GAPDH was used as an endogenous control. Relative intensity of the immunoreactive bands was analyzed, and results are shown as mean ± SD (n=3). ∗∗∗ p < 0.001, ∗∗ p < 0.01 compared with the control group. Figure S6: RP-HPLC profiles of (a) flavonoid standards and (b) flavonoid compounds in FMF at 360 nm. Peaks: 1, vitexin; 2, isovitexin; 3, isorhamnetin-3-O-β-D-glucoside; 4, narcissin.

  1. Supplementary Material