Research Article

Resveratrol Attenuates Copper-Induced Senescence by Improving Cellular Proteostasis

Figure 3

Resveratrol supplementation does not affect copper-induced expression of senescence-associated molecules. (a) Transcript relative levels of cyclin-dependent kinase inhibitor 1A (p21), apolipoprotein J (ApoJ), and transforming growth factor beta 1 (TGFβ1) were assessed by qPCR in 350 μM CuSO4-treated fibroblasts that were allowed to recover in the presence of the indicated doses of resveratrol. (b) Representative blots obtained for the determination of p21 and ApoJ protein level by western blot are depicted; the resulting densitometric analysis, normalized for control cells in the absence of resveratrol, is plotted for each analysed protein. Ponceau S staining was used to control protein loading. Data represent mean ± SEM of at least three independent experiments. ; ; and , for the comparisons between the indicated groups.
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