Research Article

circRNA_0046367 Prevents Hepatoxicity of Lipid Peroxidation: An Inhibitory Role against Hepatic Steatosis

Figure 2

circRNA_0046367 exerts antagonizing effect on miR-34a. (a) Intersection of circRNA_0046367-targeting miRNAs and hepatosteatosis-related miRNAs. (b) Complementation between MRE of circRNA_0046367 and “seed sequence” of miR-34a predicts a circRNA_0046367/miR-34a interaction. (c) Dual luciferase reporter assay displays the complementary binding of circRNA_0046367 and miR-34a. (d) Phase contrast and fluorescent microscopy for the vector transfection in HepG2 cells with FFA-induced steatosis (400x). (e) Schematic diagram for the divergent primers that are employed to amplify the circRNA_0046367 transcripts. (f) Administration of circRNA-carrying vectors induces the circRNA_0046367 normalization to exert antagonizing impact on miR-34a. The presented results are expressed as means ± S.D. .
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