Autophagy Is a Protective Response to the Oxidative Damage to Endplate Chondrocytes in Intervertebral Disc: Implications for the Treatment of Degenerative Lumbar Disc

Figure 2

H₂O₂ increases the apoptosis of endplate chondrocytes. (a) Cell viability detected by CCK-8 assay. Endplate chondrocytes were treated with 0, 100, 200, and 400 μM H₂O₂ for 24 h (). (b) The expression of Bax and Bcl-2 was examined by western blot after endplate chondrocytes were exposed to H₂O₂ (200 μM) for different times (). (c) Apoptosis incidence of endplate chondrocytes was assessed by flow cytometry after treatment with or without 200 μM H₂O₂ for 2 h (). The data are expressed as mean ± SD (; ).

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