Increasing the Fungicidal Action of Amphotericin B by Inhibiting the Nitric Oxide-Dependent Tolerance Pathway
L-NAME decreased the proliferative capacity of cells during AmB treatment, which seems independent of superoxide radical accumulation. Exponential yeast cells were treated with 10 μM AmB in the presence (white bars) or absence (black bars) of 200 mM L-NAME for 3 h. Cells were analysed for their DHE and PI fluorescence in the DMF setup (a and b) or subjected to bulk plating assays (c) every 15 min. Means and standard error of the means (SEMs) of at least 3 independent biological experiments () are presented. Two-way ANOVA followed by Tukey multiple comparison test was performed to analyse significant differences between the two treatments; Two-way ANOVA followed by Dunnett multiple comparison test was performed to analyse significant differences between the first data point (i.e., 0 min (in (c)) or 15 min (in (a) and (b))) and other data points within the same treatment (only the primary significant difference is presented to avoid overcrowding of the figure); ,, and represent ,, and , respectively. A dotted line is shown at 15 min to point out the clear differences between the responses at this time point.