Increasing the Fungicidal Action of Amphotericin B by Inhibiting the Nitric Oxide-Dependent Tolerance Pathway
The nitric oxide donor, S-nitrosoglutathione, inhibited the killing activity of AmB. Yeast cells were treated with different concentrations of AmB, in the absence (black) or presence of 200 mM L-NAME (blue) or 2 mM S-nitrosoglutathione (orange). Means and standard errors of the means (SEMs) of at least two independent biological experiments () are presented. The number of CFU/mL for different treatments (insert) was assessed by plating assays and CFU counting and is shown relative to the number of CFU/mL at the start of the experiment. Two-way ANOVA followed by Tukey multiple comparison test was performed to analyse significant differences between the two treatments; and represent and , respectively.