Research Article
Role of Protein Kinase C and Nox2-Derived Reactive Oxygen Species Formation in the Activation and Maturation of Dendritic Cells by Phorbol Ester and Lipopolysaccharide
Figure 6
Analysis of the effects of chelerythrine treatment on the formation of ROS and maturation of BM-DCs upon activation by PDBu or LPS. (a) BM-DCs (1 × 106 cells/well, 24-well plate) were stimulated with PDBu (0.1, 1, and 10 μM) and additionally treated with different concentrations of chelerythrine (10 and 100 μM), and ROS generation was measured by L-012 chemiluminescence at 20 min after stimulation. (b) ROS formation after stimulation of BM-DCs (1 × 106 cells/well, 24-well plate) with LPS (50 μg/ml) and cotreatment with chelerythrine (10 and 100 μM) was measured by L-012 chemiluminescence at 40 min after stimulation. Analysis of ROS generation (c) and maturation (d) after stimulation with LPS (1.5 μg/ml) or PDBu (10 μM) and cotreatment with chelerythrine (10–100 μM) using flow cytometry. Synthesis of 2 experiments (controls were merged) with different chelerythrine concentrations. Data are mean ± SEM of 8 (a, b) and 1-2 (c, d) experiments. versus Ctr (w/o PDBu or LPS); versus PDBu- or LPS-treated sample.
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