Neuronal Damage Induced by Perinatal Asphyxia Is Attenuated by Postinjury Glutaredoxin-2 Administration

Figure 1

Grx2 reduces neuronal damage following perinatal hypoxia/ischemia. Perinatal asphyxia was induced in rats by carotid ligation and 3 min nitrogen treatment. During 1 h postischemia, the animals were treated with recombinant Grx2 or Trx1. Hippocampi were isolated and analyzed for changes in the protein levels of the neuronal damage marker HSP70 by Western blot. The diagram depicts the relative protein levels compared to sham-operated rats that only received a saline solution (vehicle). Representative Western blots demonstrate the significant changes. Bars depict the mean + SEM of 5 rats per group. Two-way ANOVA test [condition (sham, carotid): , ; treatment (veh, Grx2, and Trx1): , ; and interaction: , ] followed by Tukey’s post hoc tests was employed to analyze the data. .