Neuronal Damage Induced by Perinatal Asphyxia Is Attenuated by Postinjury Glutaredoxin-2 Administration

Figure 2

Grx2 and Trx1 interfere with the glutamate excitotoxicity pathway following neonatal hypoxia/ischemia. Perinatal asphyxia was induced in rats by carotid ligation and 3 min nitrogen treatment. During 1 h postischemia, the animals were treated with recombinant Grx2 or Trx1. Hippocampi were isolated and analyzed for changes in the protein levels of PSD95 by Western blot, as a reporter for the induction of glutamate exitotoxicity. The diagram depicts the relative protein levels compared to sham-operated rats that only received a saline solution (vehicle). Bars show the mean + SEM of 5 rats per group. Two-way ANOVA test [condition (sham, carotid): , ; treatment (veh, Grx2, and Trx1): , ; and interaction: , ] followed by Tukey’s post hoc tests was employed to analyze the data. , .