Resveratrol Ameliorates Mitochondrial Elongation via Drp1/Parkin/PINK1 Signaling in Senescent-Like Cardiomyocytes

Figure 4

The effects of resveratrol on senescent phenotypes of H9c2 cardiomyocytes induced by D-galactose. (a) H9c2 cardiomyocytes were treated with resveratrol (RSV; 25, 50, and 100 μM) for 12 hours after 48-hour induction of D-Gal (40 g/l). SA-β-Gal activity was detected using a microscope in a bright field with positive blue staining; original magnification, 100x; the percentage of positive staining was analyzed in five randomized fields. Data () were shown as the mean ± SEM (). (b) BrdU activity was represented by BrdU (red) and DAPI (blue) double staining detected using confocal microscopy; objective magnification, 63x; scale bar represented 40 μm. The percentage of positive staining was analyzed in five randomized fields. Data () were shown as the mean ± SEM (). (c, d) H9c2 cardiomyocytes were treated with resveratrol (RSV; 25, 50, and 100 μM) for 12 hours after 48-hour induction of D-Gal (40 g/l). ROS production was evaluated by using H2DCFDA probe staining, and calcium concentration was evaluated by using Fluo-4 AM staining. Results were detected by flow cytometry. Data () were shown as the mean ± SEM (, , and ).

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