Comparative Analysis of AGE and RAGE Levels in Human Somatic and Embryonic Stem Cells under H₂O₂-Induced Noncytotoxic Oxidative Stress Conditions

Figure 2

Relative amounts of RAGE protein and mRNA gene expression in hES and somatic cells exposed to H₂O₂ for 72 h. (a–d) Relative amount of RAGE in the H₂O₂-treated cells compared with the nontreated cells. The β-actin serves as a loading control. The intensity of the bands was quantified, and relative values normalized to control condition were shown. The quantification was performed by western blot analysis: mean ± SD, 3 separate replicates per H₂O₂ experimental condition. (A–D) RAGE mRNA quantified by real-time RT-PCR in hES and somatic cells treated by noncytotoxic H₂O₂ concentrations. mRNA transcripts of RAGE were related to the amount of 18S mRNA molecules. Mean ± SD, 3 separate replicates per H₂O₂ experimental condition. Statistically significant differences between groups are indicated as follows: and as established by one-way ANOVA and Tukey’s post hoc test.