Hypoxia Downregulates MAPK/ERK but Not STAT3 Signaling in ROS-Dependent and HIF-1-Independent Manners in Mouse Embryonic Stem Cells

Figure 5

Relative expression of PP2A (a) in hypoxia in wild-type and HIF-1α −/− ES cells determined by qRT-PCR. Data are presented as mean + SEM from at least three independent experiments. Statistical significance was determined by T test. Effect of OA on signaling in hypoxia and normoxia in wild-type and HIF-1α −/− ES cells (b). Total level of β-actin was used as a loading control. Densitometry analysis of MAPK/ERK western blot expressed as fold change in RAF (S338) (c) MEK (d), and ERK phosphorylation (e). Data are presented as mean + SEM from at least three independent experiments. Statistical significance was determined by one-way analysis of variance ANOVA and post hoc Bonferroni’s multiple comparison test ().

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