Real-time deformability cytometry. (a) Real-time deformability cytometry data of a control sample was compared to cells after 60 s (b) and 120 s (c) plasma treatment. (d) The 50% and 90% density lines of each population are given for control (blue) and plasma-treated (red 60 s, green 120 s) cells. (e) After plasma treatment, melanoma cells revealed a significant increase in Young’s modulus whereas individual cell area (f) was nearly unaffected. Measurements have been carried out in a 30 μm channel at a frame rate of 2000 fps. Data shown are one representative (a–c) or mean (d) ± S.E. (e, f) of three independent experiments.