Tanshinone IIA Inhibits Glutamate-Induced Oxidative Toxicity through Prevention of Mitochondrial Dysfunction and Suppression of MAPK Activation in SH-SY5Y Human Neuroblastoma Cells

Figure 3

Effect of tanshinone IIA on glutamate-induced apoptosis in SH-SY5Y cells. The cells were treated with tanshinone IIA at the indicated concentrations followed by 10 mM glutamate exposure. The apoptotic cells were determined using Annexin V/PI staining and flow cytometric analysis (a–e). The apoptosis rate was calculated as the percentage of Annexin V-positive cells in total cells, and the data are presented as mean ± SEM of three independent experiments (f). Tan IIA: tanshinone IIA; Glu: glutamate. compared to the cells exposed to glutamate alone.

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