Tanshinone IIA Inhibits Glutamate-Induced Oxidative Toxicity through Prevention of Mitochondrial Dysfunction and Suppression of MAPK Activation in SH-SY5Y Human Neuroblastoma Cells

Table 1

Effect of tanshinone IIA on ROS level, malondialdehyde and protein carbonyl contents, and antioxidant enzymes in glutamate-exposed SH-SY5Y cells.

Treatment^a		ROS level^b	MDA content^c	Protein carbonyl content^d	Antioxidant enzyme activity^e		Antioxidant enzyme level^f
Tan IIA (μM)	Glu (mM)				SOD	CAT	SOD	CAT
0	0	52.31 ± 2.17	0.89 ± 0.04	11.18 ± 0.25	22.26 ± 0.95	133.61 ± 3.07	1.02 ± 0.02	0.39 ± 0.03
0	10	91.35 ± 6.41^g	1.21 ± 0.03^g	38.33 ± 0.49^g	14.22 ± 0.72^g	98.18 ± 3.91^g	0.71 ± 0.03^g	0.29 ± 0.01^g
2.5	10	69.12 ± 3.16^h	0.99 ± 0.05	33.69 ± 0.83^h	19.54 ± 0.61^h	113.73 ± 4.39	0.78 ± 0.02	0.30 ± 0.05
5.0	10	63.98 ± 4.07^h	0.84 ± 0.07^h	32.78 ± 0.61^h	20.21 ± 1.36^h	124.89 ± 5.44^h	0.94 ± 0.04^h	0.32 ± 0.03
10.0	10	58.59 ± 5.39^h	0.76 ± 0.04^h	30.36 ± 1.35^h	20.79 ± 1.05^h	128.42 ± 7.16^h	1.19 ± 0.07^h	0.38 ± 0.02^h

Tan IIA: tanshinone IIA; Glu: glutamate; ^apretreatment with tanshinone IIA followed by glutamate exposure; ^bROS level, relative DCF fluorescence intensity; ^cMDA content, nmol/mg proteins; ^dProtein carbonyl content, pmol/mg proteins; ^eSOD and CAT activities, U/mg proteins; ^fSOD and CAT levels, ng/mg proteins; ^g compared to the control; ^h compared to glutamate exposure.