Blockage of NOX2/MAPK/NF-κB Pathway Protects Photoreceptors against Glucose Deprivation-Induced Cell Death

Figure 10

Inhibiting the NF-κB signal protects 661W cells from GD-induced cell death. Cell death was evaluated with PI/Hoechst staining. PI-positive cells were identified with purple fluorescence and total cells were counter stained with Hoechst showing blue fluorescent nuclei. (a) 661W cells were cultured in glucose-free medium for 16 h as a vehicle control. 661W cells were treated with (1–25 μM) PDTC during the course of GD. Scale bar = 200 μm. (b) Quantitative assessment of cell death was performed through PI/Hoechst staining. Scale bar = 200 μm. Assays were performed in quadruplicate, and data are shown as means ± SEM ( one-way ANOVA and Bonferroni test, compared to the vehicle control).

(a)

(b)