Blockage of NOX2/MAPK/NF-κB Pathway Protects Photoreceptors against Glucose Deprivation-Induced Cell Death

Figure 9

Inhibition of NOX2 suppresses the activation of NF-κB signal. NF-κB expression was specifically identified with immunohistochemistry assay and nucleus was counter stained with Hoechst. (a) The cells cultured in normal medium showed that NF-κB (p65) was mainly expressed in the cytosol, which clearly showed the Hoechst stained blue nucleus. (b) The cells cultured in glucose-free medium for 16 h and the translocation of NF-κB (p65) from cytosol to nucleus was identified by the purple-colored nucleus. Scale bar = 25 μm. (c), (d) 661W cells were cultured in normal or glucose-free medium and cells were treated with 100 μM NSC or APO for 16 h. Cell lysates were collected at the indicated time periods and subjected to Western blot analysis of NF-κB (p50), phosphorylated IkBα, and nonphosphorylated IkBα. Actin protein was used as a loading control. Protein bands on the Western blots were scanned, and the intensity was determined by optical density measurements. The results are presented as means ± SEM (, compared to the control group, ^### compared to the vehicle group; one-way ANOVA and Bonferroni test).

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