BAG5 Interacts with DJ-1 and Inhibits the Neuroprotective Effects of DJ-1 to Combat Mitochondrial Oxidative Damage

Figure 1

Interaction of BAG5 with DJ-1. (a) Co-IP of overexpressed DJ-1 and BAG5. HEK293 cells were cotransfected with DJ-1-eGFP and BAG5-Myc or eGFP followed by IP with anti-GFP polyclonal antibody. In Western blot, anti-GFP antibody (Ab1218) was used for examining DJ-1 and anti-Myc antibody (#2276) for BAG5. The results showed that DJ-1 could coimmunoprecipitate BAG5. (b) Conversely, similar results were obtained by anti-Myc antibody IP (#2272). (c) Co-IP was performed using DJ-1 antibody (αDJ-1, #5933) that recognized endogenous DJ-1. In Western blot, anti-DJ-1 antibody (ab11251) was used for examining endogenous DJ-1 and anti-BAG5 antibody (ab56738) for endogenous BAG5 protein. (d) Immunofluorescence colocalization analysis of DJ-1 (green) with BAG5 (red) in HEK293 cells (above) or primary hippocampal neurons of rat transfected with DJ-1-GFP (green) and BAG5-Myc (red) (below).

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