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Oxidative Medicine and Cellular Longevity
Volume 2017, Article ID 5140360, 21 pages
Research Article

Tubulin Beta-3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary Study

1University Grenoble Alpes, ISTerre, CS 40700, 38058 Grenoble Cedex 9, France
2University Grenoble Alpes, LBFA et BEeSy, PROMETHEE Proteomic Platform, Grenoble, France
3Inserm, U1055, PROMETHEE Proteomic Platform, Grenoble, France
4CHU Grenoble Alpes, Institut de Biologie et de Pathologie, PROMETHEE Proteomic Platform, Grenoble, France
5University Grenoble Alpes, INAC, SyMMES, 38000 Grenoble, France
6CEA, INAC, SyMMES, 38054 Grenoble, France

Correspondence should be addressed to Sylvia G. Lehmann; rf.sepla-elbonerg-vinu@nnamhel.aivlys

Received 1 December 2016; Revised 1 March 2017; Accepted 19 March 2017; Published 23 May 2017

Academic Editor: Antonio Ayala

Copyright © 2017 Sylvia G. Lehmann et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Skin aging is a complex process, and a lot of efforts have been made to identify new and specific targets that could help to diagnose, prevent, and treat skin aging. Several studies concerning skin aging have analyzed the changes in gene expression, and very few investigations have been performed at the protein level. Moreover, none of these proteomic studies has used a global quantitative labeled proteomic offgel approach that allows a more accurate description of aging phenotype. We applied such an approach on human primary keratinocytes obtained from sun-nonexposed skin biopsies of young and elderly women. A total of 517 unique proteins were identified, and 58 proteins were significantly differentially expressed with 40 that were downregulated and 18 upregulated with aging. Gene ontology and pathway analysis performed on these 58 putative biomarkers of skin aging evidenced that these dysregulated proteins were mostly involved in metabolism and cellular processes such as cell cycle and signaling pathways. Change of expression of tubulin beta-3 chain was confirmed by western blot on samples originated from several donors. Thus, this study suggested the tubulin beta-3 chain has a promising biomarker in skin aging.