β-Naphthoflavone-Induced Mitochondrial Respiratory Damage in Cyp1 Knockout Mouse and in Cell Culture Systems: Attenuation by Resveratrol Treatment

Figure 3

Effects of BNF and resveratrol on mitochondrial function and ROS production in wild-type mouse liver. Complex I activity (a) and CCo activity (b) were measured in mitochondria isolated from control, BNF-, and resveratrol-treated groups ( each). ROS formation was measured in control and BNF-treated mouse liver mitochondria by Amplex Red assay system (c) using MicroWin chameleon multilabel detection platform at excitation wavelength 530 nm and emission 590 nm as described in materials and methods. (d) Blue Native Gel resolution of mitochondrial complexes from livers of control (untreated), BNF-treated and BNF plus resveratrol-treated mice. The pattern was repeated three times using extracts (80 μg each) from three separate mice for each group. Details were as described in Materials and Methods. Results represent mean ± SEM from 4 independent assays. denotes .

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