Research Article

β-Naphthoflavone-Induced Mitochondrial Respiratory Damage in Cyp1 Knockout Mouse and in Cell Culture Systems: Attenuation by Resveratrol Treatment

Figure 4

Effects of BNF and resveratrol on CcO subunit levels by immunoblot analysis. Immunoblot analysis of mitochondrial proteins from wild-type and double Cyp1a1/1a2(−/−) knockout, BNF-, and resveratrol-treated groups ( each). Mitochondrial proteins (50 μg each) were resolved by SDS-PAGE on a 12% gel and the regions of blots corresponding to CcOVb, CcOIVi1, CcOI, and SDHA were excised based on apparent molecular masses and corresponding membrane strips were subjected to immunoblot analysis with anti-CcOVb (a), anti-CcO IVi1(b), and anti-CcOI (c) antibodies. Membrane strip corresponding to ~78 kDa was probed with antibody to the mitochondrion-specific marker succinate dehydrogenase (SDHA) as loading controls. The blots were imaged through a Li-Cor Odyssey Infrared Imaging System, and the band densities were quantified using the Volume analysis software. The values in the bar diagrams represent mean ± SEM of 4 independent experiment from different mice.
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