Research Article

β-Naphthoflavone-Induced Mitochondrial Respiratory Damage in Cyp1 Knockout Mouse and in Cell Culture Systems: Attenuation by Resveratrol Treatment

Figure 8

BNF-induced ROS production in COS-7 cells stably expressing CYP1A1 protein. COS-7 cells stably expressing full-length and N-terminal truncated (+33) CYP1A1 was generated as described in Materials and Methods. (a) Immunoblot shows the level of CYP1A1 expression in the two cell lines expressing full-length and +331A1 proteins. Total cell extracts (50 μg protein each) were resolved on 12% polyacrylamide SDS gel, and the proteins were transferred to membranes as described in Materials and Methods. The blot was probed with antibody to rat CYP1A1 (1 : 1000 dilution) and β-actin. (b) ROS production as measured by Amplex Red method. Proadifen and Mito-CP were added as described in Figure 7. The immunoblot pattern in A is reproducible in multiple runs. The values in (Figure 8(b)) represent mean ± SEM of 3 independent experiments. denotes .
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