Research Article

Hederagenin Induces Apoptosis in Cisplatin-Resistant Head and Neck Cancer Cells by Inhibiting the Nrf2-ARE Antioxidant Pathway

Figure 2

Hederagenin induces apoptotic cell death in cisplatin-resistant HNC cells. (a, b) Changes in cell number and colony-forming ability in cisplatin-resistant HNC cells exposed to hederagenin (Hdg) with or without pretreatment with the antioxidant trolox (0.5 mM). NT: control cells not treated with hederagenin. (c) FACS analyses of Annexin V and propidium iodide staining of cisplatin-resistant HNC cells treated with 100 μM hederagenin. (d) Apoptosis in cells treated with various concentrations of hederagenin for 72 h was assessed by quantifying the proportion of Annexin V-positive cells. The error bars represent the standard error from three independent experiments. , relative to the control or between groups.
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