Kiwifruit Alleviates Learning and Memory Deficits Induced by Pb through Antioxidation and Inhibition of Microglia Activation In Vitro and In Vivo

Figure 5

Effects of kiwifruit treatment on Pb-induced oxidative stress in PC12 cells. (a) PC12 cells viability was assessed by MTT test after being treated with various concentrations of Pb (2.5, 5, 10, 25, and 50 μM) for 24 h. The data were analyzed by using one-way-ANOVA, followed by LSD test. (b) PC12 cells were treated with Pb, kiwifruit (Qm), and the positive control (vitamin C) for 24 h, and cell viability was assessed; We compared group of Pb, Vc, and Qm with the control group using -test for two independent samples, respectively. (c) ROS was detected with fluorescence microscope by using fluorescent probe DCFH-DA. (d) Histograms were quantitatively analyzed for changes in fluorescence intensities using the Image J software. The results are expressed as percent control. A representative experiment that was repeated at least three times. We compared group of Ctrl with Pb, Pb + Vc, and Pb + Qm using one-way ANOVA, followed by LSD test. (; ; .)

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