Mitochondrial Transfer from Wharton’s Jelly Mesenchymal Stem Cell to MERRF Cybrid Reduces Oxidative Stress and Improves Mitochondrial Bioenergetics

Figure 1

MERRF cybrid with high rate of mt.8344A>G presented higher level of ROS expression and oxidative damage. (a) Schematic drawing shows that cybrid is generated by introducing mitochondria-containing human platelet into mtDNA-depleted ρ⁰ cell. (b-c) Mt.8344A>G heteroplasmic rate was examined using PCR-RFLP. PCR fragment containing normal mt.8344A was not digested by Nae I and showed a 223 bp band. Mt.8344A>G mutation was Nae I-cleaved into 197 bp and 26 bp (not shown in gel). The proportion (%) of mt.8344A>G was quantified using ImageJ. Quantified histogram of mt.8344A>G was obtained by three independent clones. (d–f) Intracellular and mitochondrial ROS expression probed, respectively, by CM-H2DCFH and MitoSOXRed were imaged by fluorescent microscope and quantified by flow cytometry. (g) Protein carbonylation was determined using OxyBlot method. .

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