Homocysteine Induces Apoptosis of Human Umbilical Vein Endothelial Cells via Mitochondrial Dysfunction and Endoplasmic Reticulum Stress

Figure 4

Hcy induced the expression of ER stress-related proteins in HUVEC in a time-dependent manner. (a) The expression of GRP78 was determined via Western blot analysis. β-actin was selected as the loading control. (b) The expression of p-PERK and PERK was determined via Western blot analysis. (c) The expression of p-eIF2a and eIF2a was determined via Western blot analysis. (d, e) The expression of ATF4 and CHOP was determined via Western blot analysis. β-actin was selected as the loading control. (f) The expression of p-NF-κb and NF-κb was determined via Western blot analysis. The data are presented as the means ± SD of three independent experiments. , versus the 0-hour group.

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