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Oxidative Medicine and Cellular Longevity
Volume 2017, Article ID 6019796, 13 pages
Research Article

Effects of Aging and Tocotrienol-Rich Fraction Supplementation on Brain Arginine Metabolism in Rats

1Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh Campus, Sungai Buloh, Malaysia
2Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia
3Department of Anatomy, University of Otago, Dunedin, New Zealand
4School of Pharmacy, University of Otago, Dunedin, New Zealand

Correspondence should be addressed to Hanafi Ahmad Damanhuri; ym.ude.mku.mkupp@iruhnamad.ifanah

Received 21 July 2017; Revised 4 October 2017; Accepted 9 October 2017; Published 19 November 2017

Academic Editor: Anna M. Giudetti

Copyright © 2017 Musalmah Mazlan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Accumulating evidence suggests that altered arginine metabolism is involved in the aging and neurodegenerative processes. This study sought to determine the effects of age and vitamin E supplementation in the form of tocotrienol-rich fraction (TRF) on brain arginine metabolism. Male Wistar rats at ages of 3 and 21 months were supplemented with TRF orally for 3 months prior to the dissection of tissue from five brain regions. The tissue concentrations of L-arginine and its nine downstream metabolites were quantified using high-performance liquid chromatography and liquid chromatography tandem mass spectrometry. We found age-related alterations in L-arginine metabolites in the chemical- and region-specific manners. Moreover, TRF supplementation reversed age-associated changes in arginine metabolites in the entorhinal cortex and cerebellum. Multiple regression analysis revealed a number of significant neurochemical-behavioral correlations, indicating the beneficial effects of TRF supplementation on memory and motor function.