NADPH Oxidase 2 Regulates NLRP3 Inflammasome Activation in the Brain after Traumatic Brain Injury

Figure 13

Deletion of NOX2 attenuates NLRP3-TXNIP complex formation at the 7-day post-TBI in the injured mouse cortex. Representative confocal images of Duolink in situ co-IP show red fluorescence indicative of NLRP3-TXNIP complex formation in the injured cortex at the 7-day post-TBI. Mice deficient in NOX2 show reduced NLRP3-TXNIP interaction at the 7-day post-TBI in the injured cortex. Quantification of all Duolink images shows significantly increased NLRP3-TXNIP interaction after TBI that is attenuated by NOX2 deletion (presented as fold change relative to shams). mice/group. Scale bar represents 50 μM. ( sham versus WT TBI; ^#### WT TBI versus KO TBI).