mTOR Inhibition Rejuvenates the Aging Gingival Fibroblasts through Alleviating Oxidative Stress

Figure 1

The effect of rapamycin on biological characters. (a) Western blot analysis of hGFs treated with rapamycin for 72 h and 30 d. mTOR inhibition is shown by the levels of pS6. (b) Cell proliferation is detected by Cell Counting Kit-8 (CCK-8) of hGFs for 7 d after rapamycin treatment. (c) Apoptosis of hGFs is examined by an FITC Annexin V Apoptosis Detection Kit (n.s., ). (d) The cell cycle shows a modest increase in G1 fraction after rapamycin treatment (right).

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