mTOR Inhibition Rejuvenates the Aging Gingival Fibroblasts through Alleviating Oxidative Stress

Figure 3

Rapamycin delays the onset of senescence. (a) Proportion with respect to control cells and representative pictures of hGFs positive for senescence-associated β-galactosidase (SA-β-Gal) 3 days and 30 days after rapamycin treatment. (b) The results of real-time PCR show the mRNA expression of senescence-associated markers p16, p21, IL-6, and IL-8. (c) The result of Western blot shows the protein secretions of senescence-associated markers p16 and p21. (d) The results of ELISA show the cytokine secretions of senescence-associated markers IL-6 and IL-8 ( means , means , means , and means , compared with the high-passage group).

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