Mitochondria-Targeted Antioxidants SkQ1 and MitoTEMPO Failed to Exert a Long-Term Beneficial Effect in Murine Polymicrobial Sepsis
Schematic illustration of treatment, blood/tissue sampling, and monitoring. The entire study was divided into three experimental parts that followed the same treatment protocol with specific modifications described below and depicted graphically in the figure. 3-month-old female mice were subjected to polymicrobial CLP sepsis. Mice were subjected to low-volume repetitive blood sampling (30 μl blood volume/sampling; time points indicated by a single blood drop) maximally five times: At baseline (BL, immediately before CLP) 6 h, 24 h, 48 h, and 72 h post-CLP. Maximally, five intraperitoneal injections with either SkQ1, MitoTEMPO, or placebo treatment were conducted at 1 h, 12 h, 24 h, 36 h, and 48 h post-CLP (indicated by syringes). In experiment 1: mice () were monitored for 28 days (all five blood collections and treatment injections performed). In experiment 2: mice () were sacrificed at 48 h post-CLP (four blood collections and treatment injections performed). In experiment 3: mice () were sacrificed at 24 h post-CLP (three blood collections and treatment injections performed). White block arrows indicate sacrifice time point for experiments 2 and 3.
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