Ginsenoside Rg1 Ameliorates Behavioral Abnormalities and Modulates the Hippocampal Proteomic Change in Triple Transgenic Mice of Alzheimer’s Disease

Table 1

Differentially expressed hippocampus protein spots identified by 2D-DIGE/MALDI-TOF-MS/MS between the WT mice and nontreated 3xTg-AD mice.

Spot number^a	Accession number	Protein name^b	MW (Da)^c	Mascot score	3Tg versus WT
					value	Ratio^d
Modulation of synaptic transmission
5	PEBP1_MOUSE	Phosphatidylethanolamine-binding protein 1	20988	198	0.0018	1.11
15	SNP25_MOUSE	Synaptosomal-associated protein 25	23528	393	0.0074	−1.22
29	SYN2_MOUSE	Synapsin-2	63618	128	0.023	−1.19
41	CPLX2_MOUSE	Complexin-2	15499	112	0.034	−1.5
Glycolytic process
2	TPIS_MOUSE	Triosephosphate isomerase	32684	377	0.00026	−1.38
8	PGAM1_MOUSE	Phosphoglycerate mutase 1	28928	546	0.0033	−1.23
33	KPYM_MOUSE	Pyruvate kinase isozymes M1/M2	58378	52	0.027	1.47
34	ENOA_MOUSE	Alpha-enolase	47453	349	0.028	1.21
40	ALDOC_MOUSE	Fructose-bisphosphate aldolase C	39769	350	0.033	−1.08
49	ENOB_MOUSE	Beta-enolase	47337	256	0.048	1.21
50	PGK1_MOUSE	Phosphoglycerate kinase 1	44921	134	0.048	1.27
Response to stress
1	PEA15_MOUSE	Astrocytic phosphoprotein PEA-15	15102	53	0.00024	1.35
10	PDIA3_MOUSE	Protein disulfide-isomerase A3	57099	241	0.0045	1.17
18	ALBU_MOUSE	Serum albumin	70700	676	0.0086	1.24
19	VDAC1_MOUSE	Voltage-dependent anion-selective channel protein 1	32502	99	0.0089	1.11
32	TRFE_MOUSE	Serotransferrin	78841	149	0.027	1.28
45	SODC_MOUSE	Superoxide dismutase [Cu-Zn]	16104	298	0.037	1.11
52	ACTB_MOUSE	Actin, cytoplasmic 1	42052	362	0.049	−1.11
90	ANXA7_MOUSE	Annexin A7	50178	150	0.049	1.14
Negative regulation of apoptotic process
12	VDAC2_MOUSE	Voltage-dependent anion-selective channel protein 2	32340	169	0.0052	1.11
43	KCRU_MOUSE	Creatine kinase U-type, mitochondrial	47373	207	0.036	−1.2
51	COR1A_MOUSE	Coronin-1A	51641	88	0.049	−1.08
Others
3	HBA_MOUSE	Hemoglobin subunit alpha	15133	60	0.0014	−1.26
4	DPYL2_MOUSE	Dihydropyrimidinase-related protein 2	62638	441	0.0016	−1.4
6	SERA_MOUSE	D-3-Phosphoglycerate dehydrogenase	57347	115	0.002	−1.33
7	EF1A1_MOUSE	Elongation factor 1-alpha 1	50424	124	0.0021	1.28
9	DPYL5_MOUSE	Dihydropyrimidinase-related protein 5	62047	141	0.0039	1.23
11	TKT_MOUSE	Transketolase OS = Mus musculus	68272	92	0.0048	−1.22
13	GRP75_MOUSE	Stress-70 protein, mitochondrial	73701	244	0.0067	−1.29
16	WDR1_MOUSE	WD repeat-containing protein 1	67049	339	0.0078	−1.4
17	NEUG_MOUSE	Neurogranin	7720	66	0.0082	1.27
21	VATB2_MOUSE	V-type proton ATPase subunit B, brain isoform	56857	337	0.011	1.55
22	HSP7C_MOUSE	Heat shock cognate 71 kDa protein	71055	408	0.012	−1.14
24	B0R1E3_MOUSE	Histidine triad nucleotide-binding protein 1	13601	144	0.013	1.81
25	ATPA_MOUSE	ATP synthase subunit alpha, mitochondrial	59830	279	0.015	−1.14
27	HINT1_MOUSE	Histidine triad nucleotide-binding protein 1	13882	108	0.021	1.21
28	ATPD_MOUSE	ATP synthase subunit delta, mitochondrial	17589	485	0.022	1.37
30	SEP11_MOUSE	Septin-11	50005	66	0.023	1.1
35	PA1B2_MOUSE	Platelet-activating factor acetylhydrolase IB subunit beta	25736	61	0.028	1.15
36	TBA1A_MOUSE	Tubulin alpha-1A chain	50788	103	0.029	−1.2
37	FUMH_MOUSE	Fumarate hydratase, mitochondrial	54550	66	0.031	−1.14
39	ACO13_MOUSE	Acyl-coenzyme A thioesterase 13	15287	97	0.031	1.11
44	F2Z471_MOUSE	Voltage-dependent anion-selective channel protein 1	28254	216	0.036	1.16
47	GT2D1_MOUSE	General transcription factor II-I repeat domain-containing protein 1	124546	35	0.046	1.14
48	CALM_MOUSE	Calmodulin	16827	338	0.046	1.33
53	PSA2_MOUSE	Proteasome subunit alpha type-2	26024	190	0.049	1.19
67	DHSA_MOUSE	Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial	73623	192	0.025	−1.33

^aProtein ID assigned manually. ^bProtein name identified by MALDI-TOF-MS/MS. ^cTheoretical molecular weight of the proteins. ^dThe ratio in spot intensity from 3xTg-AD mice compared to WT mice. for each group.