Ginsenoside Rg1 Ameliorates Behavioral Abnormalities and Modulates the Hippocampal Proteomic Change in Triple Transgenic Mice of Alzheimer’s Disease

Table 2

Differentially expressed hippocampus protein spots identified by 2D-DIGE/MALDI-TOF-MS/MS between 3xTg-AD mice treated with and without Rg1.

Spot number^a	Accession number	Protein name^b	MW (Da)^c	Mascot score	Rg1-3Tg versus 3Tg
					value	Ratio^d
Modulation of synaptic transmission
15	SNP25_MOUSE	Synaptosomal-associated protein 25	23528	393	0.045	1.17
41	CPLX2_MOUSE	Complexin-2	15499	112	0.0016	1.66
58	SYN1_MOUSE	Synapsin-1	74223	96	0.012	−1.39
63	NSF-MOUSE	Vesicle-fusing ATPase	83131	63	0.019	−1.26
Glycolytic process
14	PGAM1_MOUSE	Phosphoglycerate mutase 1	28928	623	0.0067	1.32
33	KPYM_MOUSE	Pyruvate kinase isozymes M1/M2	58378	52	0.036	−1.12
69	ENOA_MOUSE	Alpha-enolase	47453	136	0.0092	−1.12
Response to stress
19	VDAC1_MOUSE	Voltage-dependent anion-selective channel protein 1	32502	99	0.047	−1.08
32	TRFE_MOUSE	Serotransferrin	78841	149	0.0021	−1.39
61	1433Z_MOUSE	14-3-3 protein zeta/delta	27925	151	0.01	1.19
72	CAH2_MOUSE	Carbonic anhydrase 2	29129	195	0.018	1.14
74	GLNA_MOUSE	Glutamine synthetase	42834	412	0.023	1.09
90	ANXA7_MOUSE	Annexin A7	50178	150	0.0072	−1.22
Negative regulation of apoptotic process
2	TPIS_MOUSE	Triosephosphate isomerase	32684	377	6.60E−06	1.31
18	ALBU_MOUSE	Serum albumin	70700	676	0.023	−1.24
77	GSTP1_MOUSE	Glutathione S-transferase P 1	23765	434	0.04	1.14
Others
4	DPYL2_MOUSE	Dihydropyrimidinase-related protein 2	62638	441	0.031	1.29
6	SERA_MOUSE	D-3-Phosphoglycerate dehydrogenase	57347	115	0.016	1.21
7	EF1A1_MOUSE	Elongation factor 1-alpha 1	50424	124	0.043	−1.16
24	B0R1E3_MOUSE	Histidine triad nucleotide-binding protein 1	13601	144	0.003	−1.74
56	B1AXW5_MOUSE	Peroxiredoxin-1 (fragment)	19086	379	0.00015	1.21
68	TMOD2_MOUSE	Tropomodulin-2	39487	81	0.0052	1.1
70	DHPR_MOUSE	Dihydropteridine reductase	25782	360	0.0094	1.1
73	E0CZA1_MOUSE	T-complex protein 1 subunit epsilon (fragment)	21569	49	0.019	−1.32
75	G5E8R0_MOUSE	Tropomyosin 1, alpha, isoform CRA_i	28383	275	0.034	1.15
76	GBB2_MOUSE	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2	38048	428	0.039	1.05
78	VATB2_MOUSE	V-type proton ATPase subunit B, brain isoform	56857	350	0.042	−1.93
79	ACTG_MOUSE	Actin, cytoplasmic 2	42108	176	0.049	1.08

^aProtein ID assigned manually. ^bProtein name identified by MALDI-TOF-MS/MS. ^cTheoretical molecular weight of the protein(s). ^dThe ratio in spot intensity from Rg1-treated 3xTg-AD mice compared to nontreated 3xTg-AD mice. N=6 for each group.