Semipurified Ethyl Acetate Partition of Methanolic Extract of Melastoma malabathricum Leaves Exerts Gastroprotective Activity Partly via Its Antioxidant-Antisecretory-Anti-Inflammatory Action and Synergistic Action of Several Flavonoid-Based Compounds

Table 2

Antioxidant activity and TPC value of various partitions of MEMM measured using different assay.

Sample	SOA scavenging; (%)	DPPH radical scavenging (%)	ORAC Value; (μM TE/100 g)	TPC; (mg/100 g GAE)
Sample concentration	200 μg/mL	200 μg/mL	200 μg/mL	200 μg/mL
Standard	Superoxide dismutase 6 × 10^−3 U/mL	Ascorbic acid (AA); 200 μg/mL	Trolox standard curve	Gallic acid (GAE) standard curve
PEMM	100 ± 0.0 (H)	96.29 ± 1.9 (H)	32,000 ± 3,000	279.53 ± 7.93
EAMM	98.03 ± 0.74 (H)	97.94 ± 0.4 (H)	198,000 ± 9,800	963.10 ± 35.96
AQMM	98.17 ± 1.83 (H)	98.95 ± 0.1 (H)	185,000 ± 7,300	177.57 ± 14.26

Data expressed as mean ± SEM.
SOA scavenging and DPPH radical scavenging; H: high (70–100%), M: moderate (50–69%), and L: low (0–49%).
TPC value > 1000 mg GAE/100 g is considered higher total phenolic content.
The ORAC value of triplicate wells in duplicate experiments, SEM < 20%.