Zinc Protects Oxidative Stress-Induced RPE Death by Reducing Mitochondrial Damage and Preventing Lysosome Rupture

Figure 1

Confocal images of intracellular zinc using FluoZin-3 AM live cell staining. RPE cells treated under different conditions were incubated with 5 μM FluoZin-3 AM for 30 min at 37°C in the dark. The cells were then washed and further incubated for 30 min before imaging using a confocal microscope. (a) Control RPE cells cultured in 10% serum. (b) oxPOS-treated RPE cells in 10% serum. (c) RPE cells cultured in serum-free 1.5 μM zinc. (d) RPE cells cultured in serum-free 1.5 μM zinc + oxPOS. (e) RPE cells cultured in serum-free 15 μM zinc supplement + oxPOS. (f) RPE cells cultured in serum-free 15 μM zinc supplement. (g) Control RPE cells showing autofluorescence of oxPOS. Arrows show cells with decreased intracellular zinc staining. (h) Histogram showing fluorescence intensity of FluoZin-3 AM in different treatment groups of cells, and . Data were analyzed by one-way ANOVA followed by Tukey’s multiple comparison test. .

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