Zinc Protects Oxidative Stress-Induced RPE Death by Reducing Mitochondrial Damage and Preventing Lysosome Rupture

Figure 4

In situ cell death staining to detect RPE cell apoptosis in oxPOS treated and zinc depletion. RPE cells were subjected to treatments with1.5 μM zinc, 1.5 μM zinc + oxPOS treated, oxPOS treated + 15 μM zinc supplemented, and oxPOS-treated and untreated control for 48 h were fixed and processed for TUNEL assay. (a) Control-untreated RPE cells. (b) oxPOS-treated RPE cells. (c) 1.5 μM zinc RPE cells. (d) 1.5 μM zinc + oxPOS-treated RPE cells. (e) 15 μM zinc-supplemented RPE cells. (f) oxPOS + 15 μM zinc-supplemented RPE cells. The cell nucleus is stained with DAPI (blue). Red staining indicates TUNEL positive cells. (g) Histogram showing the percentage of TUNEL positive cells in each treatment groups, and , compared to each group with the data analyzed by one-way ANOVA followed by Tukey’s multiple comparison test. 200 cells were counted for each well. .

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