Elucidation of Molecular Mechanisms of Streptozotocin-Induced Oxidative Stress, Apoptosis, and Mitochondrial Dysfunction in Rin-5F Pancreatic β-Cells

Figure 2

ROS production in STZ-induced cells. Intracellular production of reactive oxygen species was measured in control untreated and STZ-treated Rin-5F cells with different concentrations (0–10 mM) for different time intervals, using the cell permeable probe, DCFDA. Cells (~1 × 10⁵ cells/mL) were grown on cover slips and incubated with 5 μM DCFDA for 30 min at 37°C. Cells were washed twice with PBS, and fluorescence was immediately visualized using an Olympus fluorescence microscope. Representative slides from untreated control and STZ-treated cells from three experiments are shown (a). Original magnification ×200. Production of reactive oxygen species was also measured fluorimetrically in control untreated and STZ-treated cells (b). Results are expressed as mean ± SEM of three experiments. Asterisks indicate significant difference (, ) relative to the untreated control cells.

(a)

(b)