Rosmarinic Acid Alleviates the Endothelial Dysfunction Induced by Hydrogen Peroxide in Rat Aortic Rings via Activation of AMPK

Figure 5

Rosmarinic acid induced the phosphorylation of AMPK and eNOS in HAEC cells. The HAEC cells were pretreated with the AMPK inhibitor compound C (10 μM) or activator AICAR (50 μM) combined with RA (50 μM) for 10 min prior to another 10 min exposure with H₂O₂ (5 mM). The total AMPK (t-AMPK), phosphorylated AMPK at Thr172 site (p-AMPK^Thr172), and the total eNOS (t-eNOS) and the phosphorylated eNOS at the Ser1177 site (p-eNOS^Ser1177) were determined by Western blot. The results is quantified as the relative ratio of the phosphorylated protein/total protein. The values are presented as the means ± SD; . versus the untreated control group; ^## versus the H₂O₂-treated group; ^$$ versus the H₂O₂- and RA-cotreated groups.

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