Xylopine Induces Oxidative Stress and Causes G<sub>2</sub>/M Phase Arrest, Triggering Caspase-Mediated Apoptosis by p53-Independent Pathway in HCT116 Cells

<div>Effect of xylopine (XYL) in 3D in vitro model of cancer multicellular spheroids formed from HCT116 cells. (a) Cells examined by light microscopy (bar = 100 <i>μ</i>m). (b) IC<sub>50</sub> values in <i>μ</i>M and their respective 95% confidence interval obtained by nonlinear regression from three independent experiments performed in duplicate and measured by alamarBlue assay after 72 h of incubation. The negative control (CTL) was treated with the vehicle used for diluting the compound tested. Doxorubicin (DOX) and oxaliplatin (OXA) were used as positive controls.</div>

Oxidative Medicine and Cellular Longevity

fig2

Figure 2

Figure 2: Xylopine Induces Oxidative Stress and Causes G<sub>2</sub>/M Phase Arrest, Triggering Caspase-Mediated Apoptosis by p53-Independent Pathway in HCT116 Cells 

Figure 2 | Xylopine Induces Oxidative Stress and Causes G2/M Phase Arrest, Triggering Caspase-Mediated Apoptosis by p53-Independent Pathway in HCT116 Cells 