Research Article

Xylopine Induces Oxidative Stress and Causes G2/M Phase Arrest, Triggering Caspase-Mediated Apoptosis by p53-Independent Pathway in HCT116 Cells

Figure 4

Effect of xylopine (XYL) in the morphologic analysis of HCT116 cells after 24 and 48 h incubation. The cells were stained with May-Grünwald-Giemsa and examined by light microscopy (bar = 20 μm). Arrows indicated cells with a reduction in the cell volume, chromatin condensation, or fragmented DNA. The negative control (CTL) was treated with the vehicle (0.1% DMSO) used for diluting the compound tested. Doxorubicin (DOX, 1 μM) and oxaliplatin (OXA, 2.5 μM) were used as positive controls.