Xylopine Induces Oxidative Stress and Causes G₂/M Phase Arrest, Triggering Caspase-Mediated Apoptosis by p53-Independent Pathway in HCT116 Cells

Table 2

Selectivity index of xylopine (XYL).

Cancer cells	Noncancer cells
	MRC5			PBMC
	DOX	OXA	XYL	DOX	OXA	XYL
MCF7	1.4	0.3	2	4.7	2.5	1.5
HCT116	15	0.4	3.8	52	3.6	2.9
HepG2	15	1.5	2.6	52	14.9	2
SCC-9	3	N.d.	0.9	10.4	N.d.	0.7
HSC-3	5	0.5	1.5	17.3	4.5	1.2
HL-60	5	3.8	1.3	17.3	37.3	1
K-562	5	1.7	3.1	17.3	16.6	2.4
B16-F10	50	15	2.5	173.3	149	1.9

Data are presented the selectivity index (SI) calculated using the following formula: SI = IC₅₀(noncancer cells)/IC₅₀(cancer cells). Cancer cells: MCF7 (human breast carcinoma), HCT116 (human colon carcinoma), HepG2 (human hepatocellular carcinoma), SCC-9 (human oral squamous cell carcinoma), HSC-3 (human oral squamous cell carcinoma), HL-60 (human promyelocytic leukemia), K-562 (human chronic myelogenous leukemia), and B16-F10 murine melanoma. Noncancer cells: MRC-5 (human lung fibroblast) and PBMC human peripheral blood mononuclear cells. Doxorubicin (DOX) and oxaliplatin (OXA) were used as positive controls. N.d.: not determined.