Research Article

Polydatin Prevents Methylglyoxal-Induced Apoptosis through Reducing Oxidative Stress and Improving Mitochondrial Function in Human Umbilical Vein Endothelial Cells

Figure 6

PD prevents MGO-induced mitochondrial damage. HUVECs were pretreated with PD for 2 h, followed by stimulation with MGO (200 μM) for 1 h. (a) The MMP was assessed by JC-1 probe. The fluorescence intensity of JC-1 monomers (490/530 nm) and JC-1 aggregates (525/590 nm) was measured using a microplate reader. The ratio of JC-1 aggregates/JC-1 monomers was calculated. Data shown are mean ± SD for 3 independent experiments and are presented as % of control (first bar). versus Con; ## versus MGO. (b) Ultrastructural alterations of mitochondria were detected by TEM. Representative images of 3 independent experiments are shown. Distance bars: 250 nm.
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