The Synthetic Lignan Secoisolariciresinol Diglucoside Prevents Asbestos-Induced NLRP3 Inflammasome Activation in Murine Macrophages

Figure 3

Inflammasome activation following asbestos exposure leads to IL-1β and IL-18 secretion via activated caspase-1. Release of IL-1β (a) and IL-18 (c) was determined at 0, 1, 2, 4, 6, 8, 12, and 24 hours post asbestos exposure. Samples were run undiluted in triplicate, and cytokine concentrations (pg/ml) are presented as mean ± SEM. Macrophage mRNA expression of IL-1β (b) and IL-18 (d) was determined at 0, 8, and 24 hours post asbestos exposure using qPCR. Levels of target gene mRNA were normalized to 18S ribosomal RNA, and values are expressed as mean fold change from CTL at time 0. Data are presented as mean ± SEM. ^∗Statistically significant difference () between ASB- and CTL-treated cells. ^#Statistically significant difference () between ASB- and ASB + LGM2605-treated cells.

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